Exploring Small RNA Function

مهار بیان ژن GFP به وسیله تداخل RNA (RNAi) در دودمان سلولی کارسینومای جنینی P19

 Introduction: RNA interference (RNAi) is a phenomenon of gene silencing that uses double-stranded RNA (dsRNA), specifically inhibits gene expression by degrading mRNA efficiently. The mediators of degradation are 21- to 23-nt small interfering RNAs (siRNA). The use of siRNAs as inhibitors of gene expression has been shown to be an effective way of studying gene function in mammalian cells.  Ai...

Small interfering RNA; principles, applications and challenges--

Gene silencing using RNAi (RNA interference), has recently been used as a successful laboratory technique in determining the function and control of gene expression and provides a wide range of applications in molecular biology and gene therapy. RNAi is a method of suppressing gene expression. In this direction, a single-stranded RNA molecule of about 21–23 nucleotides, called siRNA (small inte...

P-44: Investigation The Role of MicroR-NAs in Spermatogenesis and Male Infertility

Background: One of the most remarkable processes during spermatogenesis is the drastic change in gene expression. It has been recently proposed that small RNAs such as micorRNAs (miRNAs) contribute a great deal of function in regulation of the gene expression in sperm. Alterations in miRNAs function prove to be detrimental to proper spermatogenesis. As many patients suffer from idiopathic infer...

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

Exploring RNA oligomerization and ligand binding by fluorescence correlation spectroscopy and small angle X-ray scattering.

RNA forms defined structures and binds specifically to target molecules. The combination of data which results from fluorescence correlation spectroscopy (FCS) and small angle X-ray scattering (SAXS) measurements can be used to determine intermolecular interactions between RNA and its binding partners. To define oligomerization states of free RNA and its complexes with bound target molecules, h...